Prochloron research

The purpose was to prepare Prochloron photosynthetic membranes for the isolation of the two major chlorophyll-proteins, the P700-chlorophyll a-protein and the light-harvesting chlorophyll a/b-protein, using SDS-polyacrylamide gel electrophoresis. The prepared proteins (purified) were examined for their cross-reactivity to polyclonal antibodies prepared from higher plant proteins. In addition, material was prepared for electron microscopy, and isolation of the DNA for determination of its general complexity (COT analysis) and similarity to barley chloroplast DNA and Anabaena DNA by using restriction-endonuclease analysis. Kleinschmidt spreads of the DNA were in the electron microscope to identify and measure the extent and size of the circlar DNA

A Comparison of Electrical Breakdown Characteristics of Composite Materials Prepared With Unmodified Micro and Nano Scale Barium Titanate

High permittivity polymer matrix composites (PMCs) have been widely researched, especially in the field of microelectronics. For this study, high permittivity materials were investigated for their potential to form part of a multi-layer electric field detector. The two main requirements for such composites were high permittivity and a dielectric strength comparable to most standard polymers used as dielectric materials. Polystyrene was selected as a host polymer due to its high dielectric strength and amorphous structure. Barium titanate, a ferroelectric ceramic from the perovskite family, was selected as a high permittivity filler. Polymer permittivity in PMCs is usually orders of magnitude lower compared to the filler permittivity, although the resultant permittivity of the composite is generally markedly lower than the permittivity of the filler may suggest. This is because very little energy is stored in the ceramic filler, such that any increase in composite permittivity is due to an increase in the average field with the polymer matrix.[1]Micro and nano scale barium titanate was blended into polystyrene in an effort to discern the initial differences between composites prepared with the two different filler types. It was found that the micro scale barium titanate was well dispersed and from studying SEM micrographs, appeared to have a good particle size distribution. The nanoscale barium titanate was found to be very poorly dispersed in polystyrene, with a wide particle size distributions formed of weakly bound aggregations and some seemingly chemically bonded agglomerations which were regular in shape with a surface texture which was indicative of tightly bound primary particles. Consistent with the differences in particle dispersion within the micro and nano composites, there was a marked difference in AC breakdown strength between the different materials. All electrical breakdown data was analysed using a 2 parameter Weibull distribution. Figure 1 compares the ? values for the micro and nano composites at different filler loadings.<br/

Applications of DFT to the theory of twentieth-century harmony

Music theorists have only recently, following groundbreaking work by Quinn, recognized the potential for the DFT on pcsets, initially proposed by Lewin, to serve as the foundation of a theory of harmony for the twentieth century. This paper investigates pcset “arithmetic” – subset structure, transpositional combination, and interval content – through the lens of the DFT. It discusses relationships between interval classes and DFT magnitudes, considers special properties of dyads, pcset products, and generated collections, and suggest methods of using the DFT in analysis, including interpreting DFT magnitudes, using phase spaces to understand subset structure, and interpreting the DFT of Lewin’s interval function. Webern’s op. 5/4 and Bartok’s String Quartet 4, iv, are discussed.Accepted manuscrip

Non-perturbative embedding of local defects in crystalline materials

We present a new variational model for computing the electronic first-order density matrix of a crystalline material in presence of a local defect. A natural way to obtain variational discretizations of this model is to expand the difference Q between the density matrix of the defective crystal and the density matrix of the perfect crystal, in a basis of precomputed maximally localized Wannier functions of the reference perfect crystal. This approach can be used within any semi-empirical or Density Functional Theory framework.Comment: 13 pages, 4 figure

Additional evidence for the migration of clay upon heating of clay–polypropylene nanocomposites from X-ray photoelectron spectroscopy (XPS)

The explanation for reduction in the peak heat release rate of polymer–clay nanocomposites which is normally accepted is that clay accumulates at the surface, forming a thermal shield which is also a barrier to mass transport. The process by which this clay arrives at the surface has never been described in print but the common assumption is that pyrolysis is required for clay accumulation to occur. In this work, X-ray photoelectron spectroscopy, a tool much more sensitive in surface analysis than conventional techniques, is used to probe the surface of polypropylene–clay nanocomposites that have been annealed at relatively low temperatures, well below that required for pyrolysis. The composition of the surface changes with time and temperature of annealing, which provide a strong indication that the clay at the surface undergoes chemical change at fairly low temperatures

Free serum haemoglobin is associated with brain atrophy in secondary progressive multiple sclerosis

Background: A major cause of disability in secondary progressive multiple sclerosis (SPMS) is progressive brain atrophy, whose pathogenesis is not fully understood. The objective of this study was to identify protein biomarkers of brain atrophy in SPMS. Methods: We used surface-enhanced laser desorption-ionization time-of-flight mass spectrometry to carry out an unbiased search for serum proteins whose concentration correlated with the rate of brain atrophy,measured by serial MRI scans over a 2-year period in a well-characterized cohort of 140 patients with SPMS. Protein species were identified by liquid chromatography-electrospray ionization tandem mass spectrometry. Results: There was a significant (p<0.004) correlation between the rate of brain atrophy and a rise in the concentration of proteins at 15.1 kDa and 15.9 kDa in the serum. Tandem mass spectrometry identified these proteins as alpha-haemoglobin and beta-haemoglobin, respectively. The abnormal concentration of free serum haemoglobin was confirmed by ELISA (p<0.001). The serum lactate dehydrogenase activity was also highly significantly raised (p<10 ) in patients with secondary progressive multiple sclerosis. Conclusions: An underlying low-grade chronic intravascular haemolysis is a potential source of the iron whose deposition along blood vessels in multiple sclerosis plaques contributes to the neurodegeneration and consequent brain atrophy seen in progressive disease. Chelators of free serum iron will be ineffective in preventing this neurodegeneration, because the iron (Fe ) is chelated by haemoglobin.This work was supported by the Wellcome Trust [100291]; Medical Research Council [MR K019090]; National Institute of Health Research; University College London Hospitals/UCL Biomedical Research Centr

The direction of tooth movement within the periodontal space. The omnidirectional transducer

KI

The direction of tooth movement within the periodontal space

KI

Relativistic Iron Lines in Galactic Black Holes: Recent Results and Lines in the ASCA Archive

Recent observations with Chandra and XMM-Newton, aided by broad-band spectral coverage from RXTE, have revealed skewed relativistic iron emission lines in stellar-mass Galactic black hole systems. Such systems are excellent laboratories for testing General Relativity, and relativistic iron lines provide an important tool for making such tests. In this contribution to the Proceedings of the 10th Annual Marcel Grossmann Meeting on General Relativity, we briefly review recent developments and present initial results from fits to archival ASCA observations of Galactic black holes. It stands to reason that relativistic effects, if real, should be revealed in many systems (rather than just one or two); the results of our archival work have borne-out this expectation. The ASCA spectra reveal skewed, relativistic lines in XTE J1550-564, GRO J1655-40, GRS 1915+105, and Cygnus X-1.Comment: to appear in the proc. of the 10th Annual Marcel Grossmann Meeting on General Relativity, 5 pages, 1 figure, uses specific .cls and .sty file

On the Robustness of NK-Kauffman Networks Against Changes in their Connections and Boolean Functions

NK-Kauffman networks {\cal L}^N_K are a subset of the Boolean functions on N Boolean variables to themselves, \Lambda_N = {\xi: \IZ_2^N \to \IZ_2^N}. To each NK-Kauffman network it is possible to assign a unique Boolean function on N variables through the function \Psi: {\cal L}^N_K \to \Lambda_N. The probability {\cal P}_K that \Psi (f) = \Psi (f'), when f' is obtained through f by a change of one of its K-Boolean functions (b_K: \IZ_2^K \to \IZ_2), and/or connections; is calculated. The leading term of the asymptotic expansion of {\cal P}_K, for N \gg 1, turns out to depend on: the probability to extract the tautology and contradiction Boolean functions, and in the average value of the distribution of probability of the Boolean functions; the other terms decay as {\cal O} (1 / N). In order to accomplish this, a classification of the Boolean functions in terms of what I have called their irreducible degree of connectivity is established. The mathematical findings are discussed in the biological context where, \Psi is used to model the genotype-phenotype map.Comment: 17 pages, 1 figure, Accepted in Journal of Mathematical Physic